Determination of Chlormequat and Mepiquat Residues in Tomato Plants Using Accelerated Solvent Extraction-Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry

Hongxia Zhang, Fengge LI, Xiaoyan LI, Xuewen LI, Weiqin YAO


An Accelerated-Solvent Extraction-Ultra performance Liquid Chromatography-Tandem Mass Spectrometry (ASE-UPLC-MS/MS) method using purified water as extraction solvent for quantitative analysis of chromequat (CQ) and mepiquat (MQ) in samples of tomato plants with higher sensibility and shorter extraction time was developed. The CQ and MQ residues and their dissipation rate were both covered in this paper. The limits of detection (S/N>3) and limits of quantitation (S/N>10) for CQ and MQ were 0.02 μg/kg and 0.1 μg/kg respectively. The linear range was 0.2~10 μg/kg and the correlation coefficients (r2) was no less than 0.9990, The average recoveries of CQ and MQ from tomato root, stem and leaf in the three spiked range of 1.0, 2.0 and 5.0 μg/kg were in the range of 100.0%~118.8% and 93.2%~110.7% respectively. The dissipation experiment showed that, on average, 98.8% of CQ residues and 99.7% of MQ residues had dissipated after 33 days, with a half-life of 3.67d and 3.66d, which can provide with guideline for using CQ and MQ on tomato in safe range.
Key words: Tomato plants; Accelerated solvent extraction; Ultra-performance liquid chromatography-tandem mass spectrometry; Chlormequat; Mepiquat


Tomato plants; Accelerated solvent extraction; Ultra-performance liquid chromatography-tandem mass spectrometry; Chlormequat; Mepiquat


ZHU, Y. H., WANG, Z. R., & LI, B. Q. (2006). Encyclopedia of agricultural chemicals. Beijing:Three Gorges Press.

JIM Vidal, Vega, A. B., & Lópezf, J. S., et al. (2004). Application of Internal Quality Control to the Analysis of Quaternary Ammonium Compounds in Surface and Groundwater from Andalusia (Spain)by Liquid Chromatography with Mass Spectrometry. J. Chromatogr A, 1050, 179-184.

FENG, S., & WANG, J. D. (2011). Determination of chlorocholine chloride and mepiquat chloride in soil and water using SPE /UPLC-MS /MS technique. Journal of Instrumental Analysis, 30(4), 439-443.

Allender, W. J. (1992). Determination of chlormequat residues in cotton seed by gas chromatography. Pestic. Sci., 35(3), 265–269.

Castro, R., Moyano, E., & Galceran, M. T. (2001). Determination of chlormequat in fruit samples by liquid chromatography-electrospray-mass spectrometry/mass spectrometry. J. AOAC Int., 84(6), 1903–1908.

Careri, M., Elviri, L., Mangia, A., et al. (2002). Rapid method for detemination of chlomequat residues in Tomato products by Ion-Exchange Liquid Chromatography-Electrospray tandem mass spectrometry. Rapid Commun in Mass Spectrom, 16(19), 1821-1826.

ZHAO, B. C., WANG, Y. L., & SUN, M. S. (2009). Application of Accelerated Extraction Solvent in Testing Analysis. Agricultural & Technology, 29(6), 85-87.

WANG, J. H., LU, X. Y., & HUANG, M., et al. (2007). Rapid screening and confirmation of chlormequat and mepiquat residues in Tomato and its products using liquid chromatography with electrospray tandem mass spectrometry. Chinese Journal of Analytical Chemistry, 35(10), 1509-1512.

Núnez, O., Moyano, E., & Galceran, M. T. (2002). Capillary electrophoresis-mass spectrometry for the analysis of quatemary ammonium herbicides. J. Chromatogr A, 974(1/2), 243-255.

Vahl, M., Gravean, A., & Juhler, R. K. (1998). Analysis of chlomequat residues in grain using liquid chromalography-mass spectrometry (LC-MS-MS). Fresenius J. Anal. Chem, 361(8), 817-820.

Commission of the European Communities. (2002). Implementing Council Directive 96/23/EC Concerning the Performance of Analytical Methods and Interpretation of Results, 2002/657/EC.2008-05-20.

Ezzell, J. L., Richter, B. E., Felix, W. D., et al. (1995). A comparison of accelerated solvent extraction for organophosphorus pesticides and herbicides. LC-GC, 13(5), 390-399.



DOI (indexed/included/archived):


  • There are currently no refbacks.

Copyright (c)

Share us to:   


How to do online submission to another Journal?

If you have already registered in Journal A, then how can you submit another article to Journal B? It takes two steps to make it happen:

1. Register yourself in Journal B as an Author

Find the journal you want to submit to in CATEGORIES, click on “VIEW JOURNAL”, “Online Submissions”, “GO TO LOGIN” and “Edit My Profile”. Check “Author” on the “Edit Profile” page, then “Save”.

2. Submission

Go to “User Home”, and click on “Author” under the name of Journal B. You may start a New Submission by clicking on “CLICK HERE”.

We only use the following emails to deal with issues about paper acceptance, payment and submission of electronic versions of our journals to databases:;;;

 Articles published in Advances in Natural Science are licensed under Creative Commons Attribution 4.0 (CC-BY).


Address: 9375 Rue de Roissy Brossard, Québec, J4X 3A1, Canada

Telephone: 1-514-558 6138
Website: Http://; Http://;

Copyright © 2010 Canadian Research & Development Centre of Sciences and Cultures