A Mini Review on Controlling the Size of Ag Nanoclusters by Changing the Stabilizer to Ag Ratio and by Changing DNA Sequence

Hua DENG, Hongtao YU


Ag nanoclusters have received considerable attention in the past decade due to their distinguished photo-physical properties, which lead to very wide potential applications for biosensing and imaging. To this point, synthesis of well-defined Ag nanoclusters for practical applications is a key issue, in particular, controlling the size (or specific number of silver atoms) of Ag nanoclusters. Herein, we briefly discuss the effect of ratio of reactants, in terms of specific functional groups, on the size of Ag nanoclusters. Also, taking DNA as an example of biopolymer, we review how the DNA sequence can affect the specific number of Ag atoms in Ag nanoclusters. These conducted principles should provide significant guidance for preparation of Ag nanoclusters of precise size.


Silver nanoclusters; Size control; Stabilizer; Ratio of stablizer to Ag; Functional groups; DNA template

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Adhikari, B., & Banerjee, A. (2010). Facile synthesis of water-soluble fluorescent silver nanoclusters and HgII sensing. Chemistry of Materials, 22(15), 4364-4371.

Antoku, Y., et al. (2010). Transfection of living HeLa cells with fluorescent poly-cytosine encapsulated Ag nanoclusters. Photochemical & Photobiological Sciences, 9(5), 716-721.

Byers, R. J., & Hitchman, E. R. (2011). Quantum dots brighten biological imaging. Progress in histochemistry and cytochemistry, 45(4), 201-237.

Chakraborty, I., et al. (2012). The superstable 25 kDa monolayer protected silver nanoparticle: Measurements and interpretation as an icosahedral Ag152 (SCH2CH2Ph) 60 cluster. Nano letters, 12(11), 5861-5866.

Chen, J., et al. (2014). A fluorescent aptasensor based on DNA-scaffolded silver-nanocluster for ochratoxin A detection. Biosensors and Bioelectronics, 57, 226-231.

Chung, H. J., et al. (2013). A magneto-DNA nanoparticle system for rapid detection and phenotyping of bacteria. Nature nanotechnology, 8(5), 369-375.

Dhanya, S., Saumya, V., & Rao, T. P. (2013). Synthesis of silver nanoclusters, characterization and application to trace level sensing of nitrate in aqueous media. Electrochimica Acta, 102, 299-305.

Díez, I., & Ras, R. H. (2011). Fluorescent silver nanoclusters. Nanoscale, 3(5), 1963-1970.

Dong, B., et al. (2013). Facile synthesis of highly photoluminescent Ag2Se quantum dots as a new fluorescent probe in the second near-infrared window for in vivo imaging. Chemistry of Materials, 25(12), 2503-2509.

Enkin, N., et al. (2014). Multiplexed analysis of genes using nucleic acid-stabilized silver-nanocluster quantum dots. ACS nano, 8(11), 11666-11673.

Gwinn, E. G., et al. (2008). Sequence‐Dependent fluorescence of DNA‐Hosted silver nanoclusters. Advanced Materials, 20(2), 279-283.

Han, S., et al. (2012). Oligonucleotide-stabilized fluorescent silver nanoclusters for turn-on detection of melamine. Biosensors and Bioelectronics, 36(1), 267-270.

Huang, Z., et al. (2011). Modulating DNA-templated silver nanoclusters for fluorescence turn-on detection of thiol compounds. Chem. Commun., 47(12), 3487-3489.

Jameson, D. M., & Ross, J. A. (2010). Fluorescence polarization/anisotropy in diagnostics and imaging. Chemical reviews, 110(5), 2685-2708.

Jia, J.-H., & Wang, Q.-M. (2009). Intensely luminescent gold (I)− silver (I) cluster with hypercoordinated carbon. Journal of the American Chemical Society, 131(46), 16634-16635.

Kumar, S., Bolan, M. D., & Bigioni, T. P. (2010). Glutathione-stabilized magic-number silver cluster compounds. Journal of the American Chemical Society, 132(38), 13141-13143.

Lan, G.-Y., Huang, C.-C., & Chang, H.-T. (2010). Silver nanoclusters as fluorescent probes for selective and sensitive detection of copper ions. Chemical Communications, 46(8),. 1257-1259.

Li, H., et al. (2012). Highly sensitive detection of proteins based on metal-enhanced fluorescence with novel silver nanostructures. Analytical chemistry, 84(20), 8656-


Liu, Y.-F., et al. (2013). Synthesis of chiral silver nanoclusters capped with small molecules. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 426(6), 12-17.

MacLean, J. L., Morishita, K., & Liu, J. (2013). DNA stabilized silver nanoclusters for ratiometric and visual detection of Hg2+ and its immobilization in hydrogels. Biosensors and Bioelectronics, 48, 82-86.

Mitrić, R., et al. (2007). Photoabsorption and photofragmentation of isolated cationic silver cluster–tryptophan hybrid systems. The Journal of chemical physics, 127(13), 134301-134301-9.

Mitrić, R., et al. (2008). Absorption properties of cationic silver cluster–tryptophan complexes: A model for photoabsorption and photoemission enhancement in nanoparticle–biomolecule systems. Chemical Physics, 343(2), 372-380.

O’Neill, P. R., Gwinn, E. G., & Fygenson, D. K. (2011). UV excitation of DNA stabilized Ag cluster fluorescence via the DNA bases. The Journal of Physical Chemistry C, 115(49), 24061-24066.

O’Neill, P. R., et al. (2009). Hairpins with poly-C loops stabilize four types of fluorescent Agn: DNA. The Journal of Physical Chemistry C, 113(11), 4229-4233.

Petty, J.T., et al. (2011). Optically enhanced, near-IR, silver cluster emission altered by single base changes in the DNA template. The Journal of Physical Chemistry B, 115(24), 7996-8003.

Petty, J. T., et al. (2013). Silver clusters as both chromophoric reporters and DNA ligands. Analytical Chemistry, 85(4), 2183-2190.

Petty, J. T., et al. (2004). DNA-templated Ag nanocluster formation. Journal of the American Chemical Society, 126(16), 5207-5212.

Petty, J. T., et al. (2013). DNA-templated molecular silver fluorophores. The journal of physical chemistry letters, 4(7), 1148-1155.

Peyser, L. A., et al. (2001). Photoactivated fluorescence from individual silver nanoclusters. Science, 291(5501), 103-106.

Qian, Y., et al. (2014). A label-free DNA-templated silver nanocluster probe for fluorescence on–off detection of endonuclease activity and inhibition. Biosensors and Bioelectronics, 51, 408-412.

Richards, C. I., et al. (2008). Oligonucleotide-stabilized Ag nanocluster fluorophores. Journal of the American Chemical Society, 130(15), 5038-5039.

Ritchie, C. M., et al. (2007). Ag nanocluster formation using a cytosine oligonucleotide template. The Journal of Physical Chemistry C, 111(1), 175-181.

Roy, S., & Banerjee, A. (2011). Amino acid based smart hydrogel: Formation, characterization and fluorescence properties of silver nanoclusters within the hydrogel matrix. Soft Matter, 7(11), 5300-5308.

Schultz, D., et al. (2013). Evidence for rod‐shaped DNA‐stabilized silver nanocluster emitters. Advanced Materials, 25(20), 2797-2803.

Sengupta, B., et al. (2008). Base-directed formation of fluorescent silver clusters. The Journal of Physical Chemistry C, 112(48), 18776-18782.

Shah, P., et al. (2014). In-solution multiplex miRNA detection using DNA-templated silver nanocluster probes. Analyst, 139(9), 2158-2166.

Shah, P., et al. (2014). Effect of salts, solvents and buffer on miRNA detection using DNA silver nanocluster (DNA/AgNCs) probes. Nanotechnology, 25(4), 045101.

Shang, L., Dong, S., & Nienhaus, G. U. (2011). Ultra-small fluorescent metal nanoclusters: Synthesis and biological applications. Nano Today, 6(4), 401-418.

Shang, L., & S. Dong, (2008). Facile preparation of water-soluble fluorescent silver nanoclusters using a polyelectrolyte template. Chem. Commun, (9), 1088-1090.

Sharma, J., et al. (2010). A complementary palette of fluorescent silver nanoclusters. Chem. Commun., 46(19), 3280-3282.

Shiang, Y.-C., et al. (2012). Fluorescent gold and silver nanoclusters for the analysis of biopolymers and cell imaging. Journal of Materials Chemistry, 22(26), 12972-12982.

Somoza, A., et al. (2013). Enhanced fluorescence of silver nanoclusters stabilized with branched oligonucleotides. Chemical Communications, (49), 4950-4952.

Soto-Verdugo, V., Metiu, H., & E. Gwinn, E. (2010). The properties of small Ag clusters bound to DNA bases. The Journal of chemical physics, 132, 195102.

Su, S., et al. (2013). iRGD-coupled responsive fluorescent nanogel for targeted drug delivery. Biomaterials, 34(13), 3523-3533.

Sun, D., et al. (2011). Anionic heptadecanuclear silver (I) cluster constructed from in situ generated 2-mercaptobenzoic acid and a sulfide anion. Inorganic chemistry, 50(24), 12393-12395.

Tsyba, I., et al. (2003). Synthesis and structure of a water-soluble hexanuclear silver (I) nicotinate cluster comprised of a “cyclohexane-chair”-type of framework, showing effective antibacterial and antifungal activities: Use of “sparse matrix” techniques for growing crystals of water-soluble inorganic complexes. Inorganic chemistry, 42(24), 8028-8032.

Vosch, T., et al. (2007). Strongly emissive individual DNA-encapsulated Ag nanoclusters as single-molecule fluorophores. Proceedings of the National Academy of Sciences, 104(31), 12616-12621.

Volkov, I.L., et al. (2013). Fluorescent Silver Nanoclusters in Condensed DNA. ChemPhys Chem, 14(15), 3543-3550.

Wang, X., et al. (2012). Luminescent fibers: In situ synthesis of silver nanoclusters on silk via ultraviolet light-induced reduction and their antibacterial activity. Chemical Engineering Journal, 210(0), 585-589.

Wang, Q.-M., et al. (2004). Intensely luminescent gold (I)-silver (I) cluster complexes with tunable structural features. Journal of the American Chemical Society, 126(31), 9488-9489.

Wu, W., et al. (2012). An Aptamer-Based Biosensor for Colorimetric Detection of Escherichia coli O157: H7. PloS One, 7(11), e48999.

Xu, H., & Suslick, K. S.(2010). Water‐Soluble fluorescent silver nanoclusters. Advanced Materials, 22(10), 1078-1082.

Yeh, H.-C., et al. (2010). A DNA− Silver nanocluster probe that fluoresces upon hybridization. Nano letters, 10(8), 3106-3110.

Yuan, J., Guo, W., & Wang, E. (2011). Oligonucleotide stabilized silver nanoclusters as fluorescence probe for drug–DNA interaction investigation. Analytica Chimica Acta, 706(2), 338-342.

Zhang, J., Xu, S., & Kumacheva, E. (2005). Photogeneration of fluorescent silver nanoclusters in polymer microgels. Advanced Materials, 17(19), 2336-2340.

Zheng, J., & Dickson, R. M. (2002). Individual water-soluble dendrimer-encapsulated silver nanodot fluorescence. Journal of the American Chemical Society, 124(47), 13982-13983.

Zhang, W., et al. (2013). In situ generation of fluorescent silver nanoclusters in layer-by-layer assembled films. Journal of Materials Chemistry C, 1(10), 2036-2043.

Zhou, J., et al. (2007). Formation and stability of G‐quadruplexes self—assembled from guanine—rich strands. Chemistry-A European Journal, 13(3), 945-949.

DOI: http://dx.doi.org/10.3968/7242

DOI (PDF): http://dx.doi.org/10.3968/g7517


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